The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran

The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran

Cytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large b...

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Main Authors: Mora, Marielos, Herrera, Alvaro, León, Pedro
Format: Online
Language:eng
Published: Universidad de Costa Rica 1996
Online Access:https://revistas.ucr.ac.cr/index.php/rbt/article/view/21568
id RBT21568
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spelling RBT215682022-05-20T15:19:43Z The genome of Epiperipatus biolleyi (Peripatidae), a Costa rican onychophoran The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran Mora, Marielos Herrera, Alvaro León, Pedro Cytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome. Cytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome. Universidad de Costa Rica 1996-04-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/21568 Revista de Biología Tropical; Vol. 44 No. 1 (1996): Volume 44 – Regular number 1 – April 1996; 153–157 Revista de Biología Tropical; Vol. 44 Núm. 1 (1996): Volumen 44 – Número regular 1 – Abril 1996; 153–157 Revista Biología Tropical; Vol. 44 N.º 1 (1996): Volume 44 – Regular number 1 – April 1996; 153–157 2215-2075 0034-7744 10.15517/rbt.v44i1 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/21568/21827 Copyright (c) 1996 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0
institution Universidad de Costa Rica
collection Revista de Biología Tropical
language eng
format Online
author Mora, Marielos
Herrera, Alvaro
León, Pedro
spellingShingle Mora, Marielos
Herrera, Alvaro
León, Pedro
The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
author_facet Mora, Marielos
Herrera, Alvaro
León, Pedro
author_sort Mora, Marielos
description Cytologic studies of Epiperipatus biolleyi, a Costa Rican onychophoran, reveal the presence of a large genome (C-value=4.3 pg/C) with many chromosomes, and a bimodal karyotype. Analysis of maje meiosis indicates lha! all chromosomes are rod-shaped, without distinct primary constrictions. Two large bivalents are consistently associated with nucleoli. Meiosis proceeds through the pachytene stage, with a few thick bivalents that break up into many small and a few large chromosomes at the diplotene stage. At metaphase the rod-shaped bivalents seem to be pulled from the telomeres into opposite poles of the spindle, lacking well defined kinetochores. DNA isolated from E. biolleyi presents a complex profile with very low GC-content in isopycnic cesium gradients. Digestion with restriction enzymes produces a homogenous spread of molecules, except with Rae III, Hind m and Bgl n, which generate distinct bands of fluorescence. A Bgl n repeat of 1.9 Kb, has been cloned for evolutionary studies. Based on these results, many repetitive DNA familíes should be found in this genome.
title The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
title_short The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
title_full The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
title_fullStr The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
title_full_unstemmed The genome of Epiperipatus biolleyi (Peripatidae), a Costa rucan onychophoran
title_sort genome of epiperipatus biolleyi (peripatidae), a costa rucan onychophoran
title_alt The genome of Epiperipatus biolleyi (Peripatidae), a Costa rican onychophoran
publisher Universidad de Costa Rica
publishDate 1996
url https://revistas.ucr.ac.cr/index.php/rbt/article/view/21568
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