Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica.
Two methods of embedding, sectioning and staining were developed to study the intemal anatomy of the delphacid planthopper Tagosodes orizicolus, one of the most important plagues of rice in Latín America and the only vector of the hoja blanca tenuiVirus (RHBV), using both light and electron rnicrosc...
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Universidad de Costa Rica
1997
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| Online Access: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/21077 |
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RBT210772022-05-25T20:44:55Z Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. Macaya-Lizano, Ana Victoria Pereira, Reynaldo Espinoza, Ana M Two methods of embedding, sectioning and staining were developed to study the intemal anatomy of the delphacid planthopper Tagosodes orizicolus, one of the most important plagues of rice in Latín America and the only vector of the hoja blanca tenuiVirus (RHBV), using both light and electron rnicroscopy. The paraffinehematoxiline- eosine Y Iliethod allows tite color identification of tissues, for example purple for fat tissue, pink for muscles, yellow- brown for exocutícle, while the resin-toluidíne-blue method preserves hetter the ultrastructure but do not permit colór identification. The informatíon obtained by these procedures is complementary and the material can also be used for in situ studies by irnmunomicroscopy, to assess the changes in cell ultrastructure and the localizatíon and replication of the RHBV during its infection cycle in the insect vector. Two methods of embedding, sectioning and staining were developed to study the intemal anatomy of the delphacid planthopper Tagosodes orizicolus, one of the most important plagues of rice in Latín America and the only vector of the hoja blanca tenuiVirus (RHBV), using both light and electron rnicroscopy. The paraffinehematoxiline- eosine Y Iliethod allows tite color identification of tissues, for example purple for fat tissue, pink for muscles, yellow- brown for exocutícle, while the resin-toluidíne-blue method preserves hetter the ultrastructure but do not permit colór identification. The informatíon obtained by these procedures is complementary and the material can also be used for in situ studies by irnmunomicroscopy, to assess the changes in cell ultrastructure and the localizatíon and replication of the RHBV during its infection cycle in the insect vector. Universidad de Costa Rica 1997-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/rbt/article/view/21077 Revista de Biología Tropical; Vol. 45 No. 2 (1997): Volume 45 – Regular number 2 – June 1997; 927–932 Revista de Biología Tropical; Vol. 45 Núm. 2 (1997): Volumen 45 – Volumen regular 2 – Junio 1997; 927–932 Revista Biología Tropical; Vol. 45 N.º 2 (1997): Volume 45 – Regular number 2 – June 1997; 927–932 2215-2075 0034-7744 spa https://revistas.ucr.ac.cr/index.php/rbt/article/view/21077/21202 Copyright (c) 1997 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0 |
| institution |
Universidad de Costa Rica |
| collection |
Revista de Biología Tropical |
| language |
spa |
| format |
Online |
| author |
Macaya-Lizano, Ana Victoria Pereira, Reynaldo Espinoza, Ana M |
| spellingShingle |
Macaya-Lizano, Ana Victoria Pereira, Reynaldo Espinoza, Ana M Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. |
| author_facet |
Macaya-Lizano, Ana Victoria Pereira, Reynaldo Espinoza, Ana M |
| author_sort |
Macaya-Lizano, Ana Victoria |
| description |
Two methods of embedding, sectioning and staining were developed to study the intemal anatomy of the delphacid planthopper Tagosodes orizicolus, one of the most important plagues of rice in Latín America and the only vector of the hoja blanca tenuiVirus (RHBV), using both light and electron rnicroscopy. The paraffinehematoxiline- eosine Y Iliethod allows tite color identification of tissues, for example purple for fat tissue, pink for muscles, yellow- brown for exocutícle, while the resin-toluidíne-blue method preserves hetter the ultrastructure but do not permit colór identification. The informatíon obtained by these procedures is complementary and the material can also be used for in situ studies by irnmunomicroscopy, to assess the changes in cell ultrastructure and the localizatíon and replication of the RHBV during its infection cycle in the insect vector. |
| title |
Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. |
| title_short |
Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. |
| title_full |
Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. |
| title_fullStr |
Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. |
| title_full_unstemmed |
Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica. |
| title_sort |
comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido tagosodes orizicolus con microscopia de luz y electrónica. |
| title_alt |
Comparación de dos métódos de preparación de tejido para estudiar la anatomía interna del delfácido Tagosodes orizicolus con microscopia de luz y electrónica |
| publisher |
Universidad de Costa Rica |
| publishDate |
1997 |
| url |
https://revistas.ucr.ac.cr/index.php/rbt/article/view/21077 |
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