Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning

Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. Lonicera japonica is a traditionally used medicinal plant, which have been recently genetically characterized by...

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Main Authors:	Yang, Luquan, Khan, Md. Asaduzzaman, Mei, Zhiqiang, Yang, Manman, Zhang, Tiandan, Wei, Chunli, Yang, Weichan, Zhu, Li, Long, Yan, Fu, Junjiang
Format:	Online
Language:	eng
Published:	Universidad de Costa Rica 2014
Online Access:	https://revistas.ucr.ac.cr/index.php/rbt/article/view/13493

id	RBT13493
record_format	ojs
institution	Universidad de Costa Rica
collection	Revista de Biología Tropical
language	eng
format	Online
author	Yang, Luquan Khan, Md. Asaduzzaman Mei, Zhiqiang Yang, Manman Zhang, Tiandan Wei, Chunli Yang, Weichan Zhu, Li Long, Yan Fu, Junjiang
spellingShingle	Yang, Luquan Khan, Md. Asaduzzaman Mei, Zhiqiang Yang, Manman Zhang, Tiandan Wei, Chunli Yang, Weichan Zhu, Li Long, Yan Fu, Junjiang Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
author_facet	Yang, Luquan Khan, Md. Asaduzzaman Mei, Zhiqiang Yang, Manman Zhang, Tiandan Wei, Chunli Yang, Weichan Zhu, Li Long, Yan Fu, Junjiang
author_sort	Yang, Luquan
description	Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. Lonicera japonica is a traditionally used medicinal plant, which have been recently genetically characterized by an improved random ampliﬁed polymorphic DNA (RAPD) analysis. In this study, the molecular markers on the basis of these RAPD fragments have been developed to identify specific L. japonica variety. The DNAs were extracted from fresh young leaves of different samples of L. japonica collected from Shenzhen, Yichang, Leshan, Emei and Loudi, China. The DNA materials were amplified using improved RAPD PCR. Different RAPD bands were excised, cloned and developed for stable sequence-characterized amplified region (SCAR) markers with different species. Two SCAR markers, JYH3-3 and JYH4-3, have been successfully cloned from improved RAPD fragments. The SCAR marker JYH3-3 was found specific for all of the L. japonica samples collected from the different regions, and another marker JYH 4-3 was strictly specific to the Shenzhen sample from Guangdong province, which is geographically distant from Hubei, Sichuan and Hunan Provinces (source of other L. japonica samples). The marker JYH3-3 was found as specific molecular marker for the identification of L. japonica, while JYH4-3 was found as molecular marker strictly specific for the Shenzhen sample. The developed SCAR markers might serve as more specific molecular markers for L. japonica variety authentication. The combination of improved RAPD analysis and SCAR marker development have resulted useful tools to study the genetic variety of any organism, which we have successfully applied here in L. japonica.
title	Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
title_short	Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
title_full	Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
title_fullStr	Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
title_full_unstemmed	Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
title_sort	development of rapd-scar markers for lonicera japonica thunb. (caprifolicaceae) variety authentication by improved rapd and dna cloning
title_alt	Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning
publisher	Universidad de Costa Rica
publishDate	2014
url	https://revistas.ucr.ac.cr/index.php/rbt/article/view/13493
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spelling	RBT134932022-06-09T17:30:45Z Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning Yang, Luquan Khan, Md. Asaduzzaman Mei, Zhiqiang Yang, Manman Zhang, Tiandan Wei, Chunli Yang, Weichan Zhu, Li Long, Yan Fu, Junjiang molecular markers Lonicera japonica random ampliﬁed polymorphic DNA sequence-characterized amplified region identification of genetic species molecular markers Lonicera japonica random ampliﬁed polymorphic DNA sequence-characterized amplified region identification of genetic species Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. Lonicera japonica is a traditionally used medicinal plant, which have been recently genetically characterized by an improved random ampliﬁed polymorphic DNA (RAPD) analysis. In this study, the molecular markers on the basis of these RAPD fragments have been developed to identify specific L. japonica variety. The DNAs were extracted from fresh young leaves of different samples of L. japonica collected from Shenzhen, Yichang, Leshan, Emei and Loudi, China. The DNA materials were amplified using improved RAPD PCR. Different RAPD bands were excised, cloned and developed for stable sequence-characterized amplified region (SCAR) markers with different species. Two SCAR markers, JYH3-3 and JYH4-3, have been successfully cloned from improved RAPD fragments. The SCAR marker JYH3-3 was found specific for all of the L. japonica samples collected from the different regions, and another marker JYH 4-3 was strictly specific to the Shenzhen sample from Guangdong province, which is geographically distant from Hubei, Sichuan and Hunan Provinces (source of other L. japonica samples). The marker JYH3-3 was found as specific molecular marker for the identification of L. japonica, while JYH4-3 was found as molecular marker strictly specific for the Shenzhen sample. The developed SCAR markers might serve as more specific molecular markers for L. japonica variety authentication. The combination of improved RAPD analysis and SCAR marker development have resulted useful tools to study the genetic variety of any organism, which we have successfully applied here in L. japonica.de cualquier organismo, que hemos aplicado con éxito en L. japonica. Genetic diversity within a species is a common feature, which plays a vital role in its survival and adaptability, and is important for the identification and authentication of a species. Lonicera japonica is a traditionally used medicinal plant, which have been recently genetically characterized by an improved random ampliﬁed polymorphic DNA (RAPD) analysis. In this study, the molecular markers on the basis of these RAPD fragments have been developed to identify specific L. japonica variety. The DNAs were extracted from fresh young leaves of different samples of L. japonica collected from Shenzhen, Yichang, Leshan, Emei and Loudi, China. The DNA materials were amplified using improved RAPD PCR. Different RAPD bands were excised, cloned and developed for stable sequence-characterized amplified region (SCAR) markers with different species. Two SCAR markers, JYH3-3 and JYH4-3, have been successfully cloned from improved RAPD fragments. The SCAR marker JYH3-3 was found specific for all of the L. japonica samples collected from the different regions, and another marker JYH 4-3 was strictly specific to the Shenzhen sample from Guangdong province, which is geographically distant from Hubei, Sichuan and Hunan Provinces (source of other L. japonica samples). The marker JYH3-3 was found as specific molecular marker for the identification of L. japonica, while JYH4-3 was found as molecular marker strictly specific for the Shenzhen sample. The developed SCAR markers might serve as more specific molecular markers for L. japonica variety authentication. The combination of improved RAPD analysis and SCAR marker development have resulted useful tools to study the genetic variety of any organism, which we have successfully applied here in L. japonica. Universidad de Costa Rica 2014-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Contribution application/pdf text/html https://revistas.ucr.ac.cr/index.php/rbt/article/view/13493 10.15517/rbt.v62i4.13493 Revista de Biología Tropical; Vol. 62 No. 4 (2014): Volume 62 – Regular number 4 – December 2014; 1649–1657 Revista de Biología Tropical; Vol. 62 Núm. 4 (2014): Volumen 62 – Número regular 4 – Diciembre 2014; 1649–1657 Revista Biología Tropical; Vol. 62 N.º 4 (2014): Volume 62 – Regular number 4 – December 2014; 1649–1657 2215-2075 0034-7744 10.15517/rbt.v62i4 eng https://revistas.ucr.ac.cr/index.php/rbt/article/view/13493/15530 https://revistas.ucr.ac.cr/index.php/rbt/article/view/13493/15531 Copyright (c) 2014 Revista de Biología Tropical http://creativecommons.org/licenses/by/4.0

Development of RAPD-SCAR markers for Lonicera japonica Thunb. (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning

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