Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro

From the germination of seeds of Euchile mariae in modified MS medium, the formation of pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and the formation of his first leaf primordium, they were used like explants to induce a morphogenetic response. Through in vitro...

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Main Authors:	Suárez-Quijada, Iris, Sandoval-Zapotilla, Estela, Hernández-Altamirano, Mabel, Chávez-Ávila, Víctor
Format:	Online
Language:	spa
Published:	Universidad de Costa Rica 2015
Online Access:	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19610

id	LANKESTERIANA19610
record_format	ojs
spelling	LANKESTERIANA196102021-06-09T20:53:37Z Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro Suárez-Quijada, Iris Sandoval-Zapotilla, Estela Hernández-Altamirano, Mabel Chávez-Ávila, Víctor orquídeas cultivo de tejidos protocormos embriogénesis somática histología histoquímica orquídeas cultivo de tejidos protocormos embriogénesis somática histología histoquí- mica From the germination of seeds of Euchile mariae in modified MS medium, the formation of pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and the formation of his first leaf primordium, they were used like explants to induce a morphogenetic response. Through in vitro culture of top and bottom protocorms sections, were obtained differentiated structures from asexual origin. Their morphology was similar to protocorms obtained from the germination of seeds, in this way we call them protocorm like bodies (PLB’s). Through of the histological analysis of these structures it was possible to reveal that these PLB’s turned out to be somatic embryos. The histochemical tests demonstrate the pres- ence of cellular contents like: proteins, lipids and starch; both in the cells of the embryos as well as in the cells of initial tissues. From the germination of seeds of Euchile mariae in modified MS medium, the formation of pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and the formation of his first leaf primordium, they were used like explants to induce a morphogenetic response. Through in vitro culture of top and bottom protocorms sections, were obtained differentiated structures from asexual origin. Their morphology was similar to protocorms obtained from the germination of seeds, in this way we call them protocorm like bodies (PLB’s). Through of the histological analysis of these structures it was possible to reveal that these PLB’s turned out to be somatic embryos. The histochemical tests demonstrate the pres- ence of cellular contents like: proteins, lipids and starch; both in the cells of the embryos as well as in the cells of initial tissues. Universidad de Costa Rica 2015-06-17 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article application/pdf https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19610 10.15517/lank.v7i1-2.19610 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 Lankesteriana: International Journal on Orchidology; 2007: Lankesteriana: Volumen 7, Número 1-2 2215-2067 1409-3871 spa https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19610/19697
institution	Universidad de Costa Rica
collection	Lankesteriana: International Journal on Orchidology
language	spa
format	Online
author	Suárez-Quijada, Iris Sandoval-Zapotilla, Estela Hernández-Altamirano, Mabel Chávez-Ávila, Víctor
spellingShingle	Suárez-Quijada, Iris Sandoval-Zapotilla, Estela Hernández-Altamirano, Mabel Chávez-Ávila, Víctor Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
author_facet	Suárez-Quijada, Iris Sandoval-Zapotilla, Estela Hernández-Altamirano, Mabel Chávez-Ávila, Víctor
author_sort	Suárez-Quijada, Iris
description	From the germination of seeds of Euchile mariae in modified MS medium, the formation of pro- tocorms was achieved. Once these reached an average size from 2 to 5 mm long and the formation of his first leaf primordium, they were used like explants to induce a morphogenetic response. Through in vitro culture of top and bottom protocorms sections, were obtained differentiated structures from asexual origin. Their morphology was similar to protocorms obtained from the germination of seeds, in this way we call them protocorm like bodies (PLB’s). Through of the histological analysis of these structures it was possible to reveal that these PLB’s turned out to be somatic embryos. The histochemical tests demonstrate the pres- ence of cellular contents like: proteins, lipids and starch; both in the cells of the embryos as well as in the cells of initial tissues.
title	Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
title_short	Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
title_full	Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
title_fullStr	Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
title_full_unstemmed	Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
title_sort	determinación histológica de regenerantes de euchile mariae (ames) withner, (orchidaceae), obtenidos a partir de protocormos cultivados in vitro
title_alt	Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro
publisher	Universidad de Costa Rica
publishDate	2015
url	https://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/19610
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_version_	1810112938898358272

Determinación histológica de regenerantes de Euchile mariae (Ames) Withner, (Orchidaceae), obtenidos a partir de protocormos cultivados in vitro

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